Part:BBa_K1688014

Nah7 - Upper naphthalene breakdown pathway (inc RBS, J23101 promoter)

Upper naphthalene breakdown pathway from the Nah7 plasmid of Pseudomonas putida + BBa_J23101 promotor and B0034 RBS. Operon consists of ten genes (NahAa, NahAb, NahAc, NahAd, NahB, NahC, NahD, NahE, NahF, NahQ) which in turn codes for seven enzymes that catalyse the breakdown of naphthalene into salicylate.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 1927
  Illegal PstI site found at 5922
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  Illegal NheI site found at 4182
  Illegal PstI site found at 1927
  Illegal PstI site found at 5922
  Illegal NotI site found at 8225
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 5275
  Illegal BamHI site found at 3260
  Illegal BamHI site found at 5962
  Illegal XhoI site found at 1371
  Illegal XhoI site found at 3759
  Illegal XhoI site found at 3911
  Illegal XhoI site found at 9413
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 1927
  Illegal PstI site found at 5922
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 1927
  Illegal PstI site found at 5922
  Illegal NgoMIV site found at 1864
  Illegal NgoMIV site found at 3639
  Illegal NgoMIV site found at 5513
  Illegal NgoMIV site found at 6125
  Illegal NgoMIV site found at 9438
  Illegal AgeI site found at 702
  Illegal AgeI site found at 3633
  Illegal AgeI site found at 6341
  Illegal AgeI site found at 8336
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 2981
  Illegal BsaI site found at 3654
  Illegal BsaI site found at 4245
  Illegal BsaI site found at 5411
  Illegal BsaI.rc site found at 2815
  Illegal BsaI.rc site found at 5565
  Illegal BsaI.rc site found at 7850
  Illegal SapI.rc site found at 3345
  Illegal SapI.rc site found at 3891
  Illegal SapI.rc site found at 7742