Part:BBa_K1859011
2(GGSGGS) linker+mRNA circularization device (3´ side)(endless)
This is Circular Parts conjuncted linker to keep folding of proteins.Circular Parts is what use sequence subcloned ribozyme involved self‐splicing in T4 Phage.By associating Circular Parts, 3'intron parts and 5'intron parts, on both ends of optional sequwnce, you can circularize mRNA making its DNA.
As for details, refer to iGEM Gifu 2014
In the study of iGEM Gifu 2014, we made long-chain protein from cyclic mRNA, but it lost functionality.It is thought that folding of the protein failed for a cause．Therefore we designed some linkers to fix the folding of the protein.These parts include sequence of Circular Parts and linker．The linker comes to be in the cyclic mRNA when mRNA is circularized.To make this device, we coupled the sequence of the linker, 2*GGSGGS , to the up stream of 5'intron.
The linker designed elsewhere is here .
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