Regulatory

Part:BBa_K1632000

Designed by: Riku Shinohara   Group: iGEM15_Tokyo_Tech   (2015-08-13)
Revision as of 08:59, 12 September 2015 by JunKawamura (Talk | contribs)

fim switch[deault ON](Tokyo_Tech/J23119)

The fim switch is the promoter containing repeated DNA sequence which is inverted by the Fim recombinase. Therefore, we can control the expression of the gene downstream of the fim switch by adding the Fim recombinase.
This fim switch has a J23119 promoter. Also between the promoter and the inverting site, there are restriction enzyme sites. Therefore, the promoter can easily be interchanged.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 345
    Illegal NheI site found at 368
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 374
    Illegal BamHI site found at 333
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//awards/part_collection/2015
//function/recombination/fim
Parameters
None