Coding
beta

Part:BBa_K1716000:Design

Designed by: Pernille Neve Myers   Group: iGEM15_DTU-Denmark   (2015-09-11)
Revision as of 21:44, 11 September 2015 by Pneve (Talk | contribs) (→‎Design Notes)


Lambda Beta recombinase optimized for expression in B. subtilis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 245


Design Notes

The coding sequence for E. coli phage (GeneBank: KT232076.1) derived lambda recombinase was optimized for expression in Bacillus subtilis using JCat.

Source

Gene product from E. coli phage lambda.

References

H. H. Wang, et al., “Programming Cells by Multiplex Genome Engineering and Accelerated Evolution,” Nature, Vol. 460, No. 7257, 2009, pp. 894-898. doi:10.1038/nature08187