File:TU Eindhoven FACS result OmpX NL.png
Revision as of 15:43, 9 September 2015 by S136969 (Talk | contribs) (FACS result of OmpX - NanoLuc, after clicking with DBCO-PEG4-TAMRA. DBCO-PEG4-TAMRA Confirmation To confirm whether OmpX is in the membrane and whether or not the non-natural amino acid is being incorporated into OmpX, DBCO-PEG4-TAMRA was used. TAMRA is )
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FACS result of OmpX - NanoLuc, after clicking with DBCO-PEG4-TAMRA.
DBCO-PEG4-TAMRA Confirmation To confirm whether OmpX is in the membrane and whether or not the non-natural amino acid is being incorporated into OmpX, DBCO-PEG4-TAMRA was used. TAMRA is a fluorescent dye that can be used to verify the “click” reaction. If the non-natural amino acid is present, DBCO-PEG4-TAMRA should “click” to the transmembrane protein OmpX and stay there. This can be analyzed with FACS. From this it can be concluded that OmpX is in the membrane and that the “click” reaction works.
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| current | 15:43, 9 September 2015 | | 1,902 × 946 (107 KB) | S136969 (Talk | contribs) | FACS result of OmpX - NanoLuc, after clicking with DBCO-PEG4-TAMRA. DBCO-PEG4-TAMRA Confirmation To confirm whether OmpX is in the membrane and whether or not the non-natural amino acid is being incorporated into OmpX, DBCO-PEG4-TAMRA was used. TAMRA is |
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