Composite

Part:BBa_K1824002

Designed by: Wenbo Xu   Group: iGEM15_XJTLU-CHINA   (2015-09-05)
Revision as of 17:08, 5 September 2015 by Registry (Talk | contribs)

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Constitutive Promoter J23119 + RNA Thermometer U6

This is a composite regulatory part that consisting of constitutive promoter BBa_J23119 and RNA Thermometer U6 BBa_K1824562 for temperature induced gene expression at 37 degrees. The possible secondary structure of U6 was simulated by RNAstructure (Fig.1). This combination is compatible with the assembly method that XJTLU-CHINA promoted.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 60
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal SpeI site found at 60
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 46
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 60
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 60
  • 1000
    COMPATIBLE WITH RFC[1000]


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