RNA

Part:BBa_K1824556:Design

Designed by: Wenbo Xu   Group: iGEM15_XJTLU-CHINA   (2015-09-04)
Revision as of 16:07, 5 September 2015 by Wenbo (Talk | contribs) (→‎Source)

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RNA Thermometer A1 (Specially designed for J23119)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To maintain the secondary structure of RNA thermometers, researchers need to be careful with of the use of any parts that couple with it. For instance, in some cases, part of operator region can be transcribed into RNAs and form tight secondary structure and thus interfere RNA thermometers.


Source

The DNA sequence of A1 was from BIT-CHINA.

References