Composite

Part:BBa_K1824000:Design

Designed by: Wenbo Xu, Hao Wu   Group: iGEM15_XJTLU-CHINA   (2015-09-04)
Revision as of 18:17, 4 September 2015 by Registry (Talk | contribs)

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Constitutive Promoter J23119 + RNA Thermometer A1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The transcription of promoters always came with redundancy, which means the last few nucleic acids of the promoter can also be transcribed. it is important to make sure that those redundant sequences do not interact with RNA thermometers.


Source

J23119 was from the Registry, and A1 was from BIT-CHINA.

References