File:2015 Freiburg WB Salmonalla gesamt.png
Revision as of 09:38, 31 August 2015 by RamonaE (Talk | contribs) (Western Blot of S. Typhimurium antigen (DHAD) and S. Typhimurium antibody (anti-DHAD, scFv). (A) Western Blot of His-tagged DHAD as well as the corresponding scFv with anti-His HRP Conjugate. The expected molecular weight of DHAD is 63 kDa and 37 kDa for )
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Western Blot of S. Typhimurium antigen (DHAD) and S. Typhimurium antibody (anti-DHAD, scFv). (A) Western Blot of His-tagged DHAD as well as the corresponding scFv with anti-His HRP Conjugate. The expected molecular weight of DHAD is 63 kDa and 37 kDa for the scFv, respectively. (B) Western Blot of DHAD with the purified S. Typhimurium scFv was used in a 1:100 dilution. Additionally, the scFv is c-Myc tagged. Anti-c-Myc antibody (1:1000; rabbit) was used in a second step. For detection, the anti-rabbit HRP antibody (1:5000) was used.
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| current | 09:38, 31 August 2015 | | 963 × 425 (99 KB) | RamonaE (Talk | contribs) | Western Blot of S. Typhimurium antigen (DHAD) and S. Typhimurium antibody (anti-DHAD, scFv). (A) Western Blot of His-tagged DHAD as well as the corresponding scFv with anti-His HRP Conjugate. The expected molecular weight of DHAD is 63 kDa and 37 kDa for |
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