Part:BBa_K1321367

sfGFP + linker-CBDcipA-linker with T7 promoter

A T7-promoter expression construct of super-folder GFP fused N-terminally to CBDcipA (a cellulose-binding domain), which contains an endogenous N and C-terminal linker sequence.

This construct is part of a library of Super-folder GFP fusions with cellulose binding domains, which we used to assay the CBD binding affinity. Please see our [http://2014.igem.org/Team:Imperial/Functionalisation project page] for more information. The collection of sfGFP-CBD fusion parts can be seen in the table below:

Note that the stop codon plus 6 bp at the end of the sequence are included the RFC25 suffix which is not shown. The prefix to this part is RFC10 format.

For reference the cellulose binding domain binding capability to bacterial cellulose was measured relative to other cellulose binding domains when fused to sfGFP, the data for which can be seen here - K1321371.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 923
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 923
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 923
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 923
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 923
  Illegal NgoMIV site found at 53
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 68