Part:BBa_K1523021

nahR coding sequence,encoding a LysR-type transcriptional regulator

nahR, encoding a LysR-type transcriptional regulator, is highly conserved among naphthalene-degrading bacteria isolated from a coal tar waste-contaminated site and in extracted community DNA. It contains a kind of HTH LysR DNA-binding domain.

NAH7 plasmid specify catabolism of naphthalene and salicylate under positive regulation by gene nahR. A 1.75-kb fragment (PstI-HindIII) cloned into the pCP13 derivative of vector RK2 complemented in trans five nahR mutations. The fragment sequence contained a 1,122-base-pair open reading frame with a predicted sequence of 374 residues that was rich in basic amino acids with regions similar to known DNA-binding proteins. Clones from the nahR gene region were expressed in mexicells. Plasmid pY1923, carrying the 1.75-kb PstI-HindIII fragment, expressed a protein of Mr ca. 35,000 which bound to the upstream region of gene nahR in a gel electrophoresis DNA-binding assay. Other clones expressed proteins of currently unknown function; pY1311, with the 1.1-kb HindIII fragment, produced a polypeptide with an Mr of 23,000, and pY1812, with the 1.2-kb PstI-SphI fragment, produced a polypeptide (Mr 41,000) which appeared to be a fused nahR-lacZ product.

Operons repressed by nahR can be regulated positively by nahR when salicylate appears.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 57
  Illegal PstI site found at 250
  Illegal PstI site found at 469
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 57
  Illegal PstI site found at 250
  Illegal PstI site found at 469
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 323
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 57
  Illegal PstI site found at 250
  Illegal PstI site found at 469
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 57
  Illegal PstI site found at 250
  Illegal PstI site found at 469
  Illegal NgoMIV site found at 491
  Illegal NgoMIV site found at 646
  
• 1000
  COMPATIBLE WITH RFC[1000]