Part:BBa_K1442040
MS2 bacteriophage coat protein
Regulating the expression of a protein can be achieved through repressive elements; we have the MS2 and MS2 hairpin, which regulate the production of RNA Dependent RNA polymerase (RdRp). As RdRp is fused with MS2 through a P2A linker, an indefinite increase in RdRp production is rate-limited by MS2 production. MS2 is able to bind to the MS2 hairpin sequence, also rate-limiting RdRp production, which in turn regulates the whole replicon system. The iGEM community can benefit from a regulation system, in which MS2 can be fused to a protein of interest to regulate its expression. If the fusion protein is non-functional, engineering restriction sites in the P2A linker and site specific ligation reconstitutes RdRp without the MS2 hairpin. The part derives from Auslander et al, which showed that the MS2 protein-box relationship can be designed for use in synthetic biology to create programmable single-cell mammalian biocomputers with simple expression logic, increasing the complexity of an information processing system without researcher input.
Usage and Biology
The MS2 coat-protein was included in our replicon.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 255
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 255
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 255
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 255
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 255
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 292
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