Coding

Part:BBa_K1321113:Design

Designed by: Xenia Spencer-Milnes   Group: iGEM14_Imperial   (2014-10-09)
Revision as of 20:18, 24 October 2014 by Gabi1234 (Talk | contribs) (→‎Source)


Phytochelatin (PC) EC20 + linker-CBDcipA-linker with LacI promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 512
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 512
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 512
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 512
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 512
    Illegal NgoMIV site found at 230
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

PhytoC+CBD10 with LacI


Source

Phytochelatin and CBDcipA with its linker were synthesized from Geneart and cloned into the psB1C3 backbone. The LacI part was made by inverse PCR of BBa_J04500, and ligated.

References