Part:BBa_K1415202

Pcons+B0034+PBAN(Mamestra brassicae)+B0034+BFP+J61048

To predict the PBAN expression in E.coli by computer modeling, we next tested PBAN BFP biobricks. We obtained the average expressive value of the blue fluorescence in the biobrick part (above) and also the control part of Pcons + RBS + BFP + Ter. Therefore, we can use the average value to generate predictions of the PBAN expression in E.coli.

Fig.1-1 Biobrick of Pcons + RBS + PBAN(MB) + BFP + Term.

Fig.1-2 The bluelight test of our E.coli

Fig.1-3-1 The blue light fluorescence expression curve of E.coli containing Pcons + RBS + PBAN(MB) + RBS + BFP + Ter plasmid (control: competent cells that cannot emit blue light).

Fig.1-3-2 The growth curves of PBAN(MB)

Modeling

Fig.1-4 Modeling result of Pcons + RBS + PBAN(MB) + BFP + Ter. The blue line is the expression profile of the theoretical biobrick. And the green line is the expression data of Pcons + RBS + PBAN(MB) + BFP + Ter. And the red line is the adjusting line from the green and blue one. This line represent the correcting line of theoretical data and real condition data which can make our model not only fit the theoretical condition but also stay away from experimental bias.

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 79
  Illegal NgoMIV site found at 909
  
• 1000
  COMPATIBLE WITH RFC[1000]