Generator

Part:BBa_J36851

Designed by: Perry Tsai   Group: iGEM06_Harvard   (2006-10-29)
Revision as of 21:23, 29 October 2006 by Registry (Talk | contribs)

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Lac-inducible generator of Lpp-OmpA(46-159)-Streptavidin single-chain dimeric + His6 tag

This part codes for the core part of wild-type streptavidin protein, without the start codon. Streptavidin protein binds strongly to the biotin molecule, and can be used to bind biotinylated nucleic acids or peptides. However, this version has been mutated so that it will not bind biotin strongly.

It was used as part of a fusion protein with lipoprotein signal peptide and outer membrane protein A transmembrane domains, to express streptavidin on the outer surface of E. coli. This dead mutant would serve as a type of negative control.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 1458
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1467
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 888
    Illegal AgeI site found at 1422
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None