Generator

Part:BBa_K1448008

Designed by: yuma kobayashi   Group: iGEM14_KIT-Kyoto   (2014-10-16)
Revision as of 05:49, 18 October 2014 by UMBABA (Talk | contribs)

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In E coli mono-terpene production cassette

This part contains the IPTG inducible tac promoter, RBS and erg20-2 generator (BBa_K1448007).

Usage and Biology

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How to use

First, digest this part with SpeI.


Second, digest a BioBrickPart encoding a monoterpene synthase with SpeI and XbaI.


Third, mix and ligate both samples.


Various monoterpenes can be produced in E. coli cells containing this device and monoterpene synthase genes without exogenous GPP.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 126
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None