Generator

Part:BBa_J36850

Designed by: Perry Tsai   Group: iGEM06_Harvard   (2006-10-29)
Revision as of 21:18, 29 October 2006 by Registry (Talk | contribs)

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Lac-inducible generator of Lpp-OmpA(46-159)-Streptavidin wild-type + His6 tag

This part codes for the core part of wild-type streptavidin protein, without the start codon. Streptavidin protein binds strongly to the biotin molecule, and can be used to bind biotinylated nucleic acids or peptides.

There is a histidine tag added at the end of the coding sequence right before the stop codons. You can probe for this protein with an anti-his(C-term) antibody.

It was used as part of a fusion protein with lipoprotein signal peptide and outer membrane protein A transmembrane domains, to express streptavidin on the outer surface of E. coli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 711
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 753
    Illegal AgeI site found at 804
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None