Composite

Part:BBa_K1520020

Designed by: yue wu   Group: iGEM14_Nanjing-China   (2014-09-22)
Revision as of 01:24, 18 October 2014 by Sagyman (Talk | contribs)

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Pcons2-rbs-lacI-Ter-Plac-rbs-luxI-Ter-Pcons2-rbs-luxR-Ter

Pcons2-rbs-lacI-Ter-Plac-rbs-luxI-Ter-Pcons2-rbs-luxR-Ter

It produces luxR-AHL complex when there is enough IPTG.

K1520020.jpg

From left to right, Marker(1Kb), Pcons2-rbs-lacI-T-Plac-rbs-luxI-T, Pcons2-rbs-lacI-T-Plac-rbs-luxI-T-Pcons2-rbs-luxR-Ter. The substances of electrophoresis are made from PCR of plasmid with VF2 and VR as their primers.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 2380
    Illegal NheI site found at 2403
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1208
    Illegal BglII site found at 2222
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds
//cds/transcriptionalregulator/activator
//cds/transcriptionalregulator/repressor
//function/cellsignalling
//function/regulation/transcriptional
Parameters
biology
controlIPTG, lacI
directionForward
ec_numnone
functionMixed
keggnone
ligandsHSL
n/aconstitutive promoter family member
negative_regulators
o_h
o_l
positive_regulators
proteinLuxR
swissproP12746
tagNone
uniprotP48445