Part:BBa_K1431812:Experience
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how you used this part and how it worked out.
Applications of BBa_K1431812
After construction, this part was transformed into BL21 & DH5α to test expression efficiency. In the meantime, we made a comparison between BBa_K1431812 and a part whose promoter is to BBa_J23106(weak).
We spread plate at 10:00 on 15th, Oct. Following are pictures which were taken several hours after placing the plates into a incubator.
JA equals to J23100, and JB equals to J23106.
At 00:30 (16th,Oct.)
At 1:50 (16th,Oct.)
At 3:00 (16th,Oct.)
At 6:00 (16th,Oct.)
At 7:40 (16th,Oct.)
At 8:00 (16th,Oct.)
At 10:00 (16th,Oct.)
At 11:20 (16th,Oct.)
Then we isolated single colonies from the plates.
At 00:50 (17th,Oct.)
At 02:45 (17th,Oct.)
At 04:55 (17th,Oct.)
At 22:40 (17th,Oct.)
With observation, expression in BL21 is stronger than that in DH5α, and the part with promoter J23106 unexpectedly is stronger.
User Reviews
UNIQ09591f35b0c4533d-partinfo-00000000-QINU UNIQ09591f35b0c4533d-partinfo-00000001-QINU