Featured Parts:RNA-Lock-and-Key

Revision as of 05:10, 27 April 2006 by Smelissali (Talk | contribs) (→‎Background: How the Riboregulator Works)

The Parts

Bba_J01008 - ("key 1") Biobricked version of Isaacs' riboregulator trans activating key, taR12
Bba_J01010 - ("lock 1") Biobricked version of Isaacs' riboregulator cis repressed lock, crR12.
Bba_J01080 - ("lock 3") Modified version of J01010
Bba_J01086 - ("key 3") Modified version of J01008

Background: How the Riboregulator Works

This set of riboregulators are based upon the work of Isaacs et al 2004 (see reference #1). They work by repressing the expression of a target gene by introducing a complementary region to prevent transcription. The DNA is modified directly upsteam of the target gene's ribosome binding site (RBS) such that the 5' untranslated region will fold to form a stem-loop secondary structure, thus blocking the RBS and preventing transcription. This stem-loop structure is known as the 'cis-repressed' mRNA (crRNA)

Suggested Uses for these Biobricks

Experimental and Technical

Technical

Previous Experiments

References

  • Publications on the Isaacs Riboregulator:
    • Isaacs, Dwyer DJ, Ding C, Pervouchine DD, Cantor CR, Collins JJ. 2004. "Engineered riboregulators enable post-transcriptional control of gene expression". Nature Biotechnology 2004. [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=15208640&query_hl=7&itool=pubmed_docsum Pubmed] [http://www.nature.com/nbt/journal/v22/n7/abs/nbt986.html;jsessionid=ABB92F041E994E3B8E4A5213699C3CB0 Journal]
  • More parts and resources
    • A listing of more parts made by the Berkeley 2005 iGEM team including constituitively 'on' promoter-controlled riboregulators and other experimental constructs can be found here