Coding

Part:BBa_J34802:Design

Designed by: Alexandra Choutko   Group: iGEM06_ETHZ   (2006-10-26)
Revision as of 08:57, 27 October 2006 by Choutkoa (Talk | contribs) (→‎References)

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CI repressor fusion, transcription factor


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 463
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

cI lambda repressor fused via TVMV cleavage site to alpha subunit of RNAP NTD

Source

References

Sreedevi Nallamsetty et al., Efficient site-specific processing of fusion proteins by tobacco vein mottling virus protease in vivo and in vitro, Protein Expression and Purification 38 (2004) 108–115 Rachel B. Kapust and David S. Waughn, Controlled Intracellular Processing of Fusion Proteins by TEV Protease, Protein Expression and Purification 19, 312–318 (2000)