Part:BBa_K660004:Experience

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••• Exeter University 2014

As part of the Exeter 2014 project iLOV was used as a reporter in two constructs. iLOV was used to test the effectiveness of a NemR upstream intergenic region (https://parts.igem.org/Part:BBa_K1398005) in part BBa_1398004 (https://parts.igem.org/Part:BBa_K1398004). Part BBa_1398007 (https://parts.igem.org/Part:BBa_K1398007) used iLOV to test the effectiveness of a NemR recognizing promoter, part BBa_1398008 (https://parts.igem.org/Part:BBa_1398008). (Full article available at:http://2014.igem.org/Team:Exeter/Detection#TheNemRpromoterconstruct%28BBa_K1398007%29) As part of their project the fluorescence topography of iLOV was mapped. The response of iLOV to a low power light source was also explored. The fluorescence response of iLOV was greatest when excited with photons of wavelength 449±1nm whilst photon emissions where detected at a wavelength of 494±2nm. (Full article available at: http://2014.igem.org/Team:Exeter/iLOVCharacterisation)

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