Part:BBa_K1321305
Gluconacetobacter xylinus ATCC 53582
Gluconacetobacter xylinus is an obligate aerobe, Gram negative bacterial species which produces bacterial cellulose. Strain ATCC 53582 has been reported as one of the highest cellulose-producing strains, and readily produces cellulose with different carbon feedstocks.
G.xylinus ATCC 53582 genome sequence
G.xylinus ATCC 53582 genome was sequenced as a part of Imperial iGEM 2014 project. The genome is approximately 3.5Mbp in size.
Culturing G. xylinus ATCC 53582
G.xylinus ATCC 53582 growth rate varies depending on the culturing conditions. Growth rate is highest when G.xylinus is cultured at 30degC, 180rpm shaking in a well-aerated setting. However, several authors have noted that in shaking conditions, the prevalence of cellulose non-producing mutants (cel- mutants) increases, which causes a decrease in the total yield of produced cellulose. For this reason, when high cellulose production is required, G.xylinus is often grown in static conditions, at 30degC, in a well-aerated setting. In shaking cultures, ATCC 53582 reaches stationary phase in 48-72 hours, whereas in static cultures, depending on the volume of the medium, it can take up to 10 days. When grown on HS-agar plates at 30degC, colonies become visible within 48-72 hours. Although several different media have been used for culturing G.xylinus, the most commonly used is Hestrin-Schramm (HS) medium. HS medium contains the following components (for a protocol for G.xylinus culturing, see (G.xylinus media and culturing on Imperial 2014 wiki): 2% (w/v) glucose 0.5% (w/v) yeast extract 0.5% (w/v) peptone 0.27% (w/v) Na2HPO4 0.15% (w/v) citric acid
Cellulose productivity of G.xylinus ATCC 53582
ATCC 53582 has been reported as one of the highest cellulose-producing strains (see Figure 1 for comparison of ATCC53582 and KI strains).
File:IC14 ATCC and KI Cellulose productivity
Feedstocks for G.xylinus ATCC 53582
G.xylinus is commonly grown on HS-glucose. However, it can readily use other carbon feedstocks, including sucrose and glycerol. have found that ATCC 53582 cellulose productivity is highest when grown on glycerol (see Figure 2.)
File:IC14 ATCC and KI carbon feedstocks experiment
Natural antibiotics resistance of G.xylinus ATCC 53582
See Figure 3.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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