Reporter

Part:BBa_J04430:Experience

Designed by: Kristen DeCelle   Group: iGEM2005   (2005-06-09)
Revision as of 18:57, 15 October 2014 by CarriedC (Talk | contribs)


Applications of BBa_J04430

Warwick iGEM 2014 used this part as a positive control for our experiments investigating promoter strengths for a self replicating RNA strand, using the Hepatitis C Virus NS5B gene encoding RNA dependent RNA polymerase, as the replicating enzyme.

This was measured using a tecan Magellan plate reader in which fluorescence in the GFP range was measured over a period of 24 hours with both biological and technical replicates for each transformed cell type.

Results

User Reviews

UNIQa68a0a49886b8edb-partinfo-00000001-QINU

No review score entered. arandall

1 mM IPTG induced expression using BL21 E. coli cells produces vibrant green cells from both 08 and 09 distributions.

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Smelissali

A lovely vibrant green under UV --Smelissali 19:45, 3 July 2006 (EDT)

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Antiquity

This review comes from the old result system and indicates that this part worked in some test.

UNIQa68a0a49886b8edb-partinfo-00000005-QINU