Part:BBa_K1493000

Fusaric acid induced regulatory promoter

Promoter fusaric acid inducible

Usage and Biology

A fusaric acid efflux pump within Pseudomonas putida is encoded by an operon consisting of four genes. This operon is controlled by a LysR-type gene (pp1262) which is located upstream of the operon. This gene inhibits the binding of RNA polymerase to the promoter in the intergenic region between pp1262 and the operon. Fusaric acid will block this inhibition allowing activity of the operon. (See figure 1) Hence, pp1262 and the intergenic region will be isolated and put into BioBrick form, effectively acting as a Fusaric Acid inducible Promoter (FAiP).
Figure 1. Fusaric acid efflux pump operon present in the genome of KT2440 Pseudomonas putida.

Features

This biobrick can only be used in Pseudomonas putida, since the regulatory gene has been disrupted. In P. putida, the suppressing gene is still expressed, which will still inhibit the binding of the RNA polymerase to this part, resulting in a functioning biobrick. This disruption will also stop overexpression of the inhibiting gene, which also inhibits the efflux pump in the original genome and thus decreases its host's immunity to fusaric acid. The biobrick with an intact inhibitor gene can be found here: https://parts.igem.org/Part:BBa_K1493001

References

Hu, R.-M., et al., An Inducible Fusaric Acid Tripartite Efflux Pump Contributes to the Fusaric Acid Resistance in Stenotrophomonas maltophilia. PLoS ONE, 2012. 7(12): p. e51053. Nelson, K.E., et al., Complete genome sequence and comparative analysis of the metabolically versatile Pseudomonas putida KT2440. Environmental microbiology, 2002. 4(12): p. 799-808.