Part:BBa_K1537025

FDH

Formate dehydrogenase is a mitochondrial-localized NAD-requiring enzyme while the HCOOH is getting into the mitochondrial，FDH will oxidize the formic acid into CO2, and reduce NAD+ to NADH with a high degree of specificity.In our project, the heterologous expression of FDH from arabidopsis thaliana in tobacco was completed.

The abbreviations are as follows: CAT:catalase;FALDH:glutathione-dependent formaldehyde dehydrogena-se;FGH: S-formylglutathione hydrolase;FDH: Formate dehydrogenase;SYN: 10-Formyl-THF synthetase;FTD: 10-formyltetrahydrofolate dehydrogenase MTD: 5,10-methylenetetrahydrofolate dehydrogenase;MTC: 5,10-methylenetetrahydrofolate cyclohydrolase;SHMT: Serine hydroxymethyl transferase;GDC: Glycine decarboxylase complex;GXS: Glyoxalic acid synthetase;GXDC: Glyoxalic acid decarboxylase;HM-GSH: S-Hydroxymethyl glutathione;Forml-GSH: Formyl glutathione;SMM cycle: Methionine cycle.

References

Zhang Wei.（2012）The studies on formaldehyde metabolic mechanism in Petunia hybrida and the genetic manipulation for enhancing its formaldehyde phytoremediation ability. A dissertation submitted for the degree of Master of Science at theKUNMING UNIVERSITY OF SCIENCE AND TECHNOLOGY.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]