Plasmid

Part:BBa_K1321304:Design

Designed by: Michael Florea   Group: iGEM14_Imperial   (2014-10-08)
Revision as of 11:36, 10 October 2014 by Mf1512 (Talk | contribs) (→‎Source)


pSEVA321-BB with J23117-RFP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 4476
    Illegal SpeI site found at 37
    Illegal PstI site found at 920
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4476
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal SpeI site found at 37
    Illegal PstI site found at 920
    Illegal NotI site found at 913
    Illegal NotI site found at 4482
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4476
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 4476
    Illegal SpeI site found at 37
    Illegal PstI site found at 920
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 4476
    Illegal XbaI site found at 4491
    Illegal SpeI site found at 37
    Illegal PstI site found at 920
    Illegal NgoMIV site found at 2095
    Illegal NgoMIV site found at 2980
    Illegal NgoMIV site found at 4091
    Illegal NgoMIV site found at 4215
    Illegal AgeI site found at 616
    Illegal AgeI site found at 728
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

y


Source

The original pSEVA321 backbone comes from the SEVA (Standard European Vector Architecture) collection.

References