Regulatory

Part:BBa_K1349003:Design

Designed by: Alexia Satouf, Clara Bouyx, Aimeric Agaoua, Lambert Antoni, Vincent Castel   Group: iGEM14_Aix-Marseille   (2014-10-06)
Revision as of 07:36, 9 October 2014 by LISM JS (Talk | contribs) (→‎References)

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PcusC


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

CusR is a response regulator that binds to DNA when phosphorylated. This small part is the sensitive CusR promoter from E. coli and we designed it to be a promoter positively regulated by CusR phosphorylation. In common with many response regulators we expect over-expression of CusR to result in binding and so positive regulation of the promoter. This part is complementary to part BBa_K1349002.

A construct with this part downstream of a gene is expected to show positive regulation of the gene by CusR phosphorylation (or CusR overexpression).

Source

The part was amplified by PCR from the pWW2181 plasmid described by Whitaker et al.

References

Whitaker WR, Davis SA, Arkin AP, Dueber JE. 2012. Engineering robust control of two-component system phosphotransfer using modular scaffolds. Proc Natl Acad Sci U S A. 109(44):18090-5. doi: 10.1073/pnas.1209230109.