Part:BBa_K1413021

bphR2 mutated

bphR2 gene, from Pseudomonas pseudoalcaligenes KF707, encodes for a regulatory protein (bphR2) which can detect polychlorinated biphenyls (PCBs). This gene regulates negatively bphR1 promoter involved in the transcription of biphenyl-catabolic genes (bph), degrading PCBs. In absence of PCB, bphR2 is bound to the bphR1 promoter. When compound diffuses into the cell, it binds to protein allowing a conformational change. BphR2 is release from the promoter that its allows the transcription of bph genes and therefore the degration of PCBs.

Our aim was to finish and characterize the part of 2013 Paris Saclay's team. We used bphR2 in our biosensor project of PCBs. Instead of the genes of degradation, we have RFP (BBa_E1010) to detect 2 hydroxy-3',4' biphenyl.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 475
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 259