Reporter
rev RFP

Part:BBa_K1463520:Design

Designed by: Beth Greig   Group: iGEM14_Glasgow   (2014-08-12)
Revision as of 10:28, 2 October 2014 by Scolloms (Talk | contribs) (→‎Source)


E1010 RFP in reverse


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 34
    Illegal AgeI site found at 146
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

No specific design considerations other than ensuring that the sequence was in reverse.


Source

Made by PCR using E1010 as a template and primers XXXX and YYYYY to incorporate prefix at the end and suffix at the beginning.

References