Measurement

Part:BBa_K1379005:Design

Designed by: Jordy Evan, Nadia Benedicta, Angeline Widjaja   Group: iGEM14_Hong_Kong_HKUST   (2014-09-23)
Revision as of 16:58, 23 September 2014 by Ngsiuwang (Talk | contribs) (→‎Source)


σx Generator + PcelA-E0240


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 359
    Illegal BsaI.rc site found at 1490


Design Notes

N/A


Source

Both comX gene and PCelA are found in genomic sequence of Streptococcus pneumoniae NCTC7465 strain, while the RBS+GFP+DTT, downstream of PCelA, is BBa_E0240. The promoter encoding comX is BBa_K880005 and the double terminator for it is BBa_B0015

References