Frequently Used Parts

Revision as of 22:23, 31 March 2014 by Kim (Talk | contribs)

The iGEM Registry has a vast collection of parts that have been contributed over the course of our 10 year history. We have more than 20 thousand parts that iGEMers, graduate students, postdocs, PIs and even high schoolers have contributed to the collection.

In the beginning of iGEM, it was difficult to get experiments to work as expected. As the competition matured and labs became more used to making and testing BioBricks, more things worked, but not much documentation was contributed. Finally, we required iGEMers to submit highly-documented parts to be eligible for medals. This may not seem like a big change for a single team, but we're building the Registry for future teams.

The job or every iGEMer and contributor to the Registry is to make their part as awesome as possible to inspire students to use it in following years. With over 20,000 parts already in our collection, this is not an easy feat. We encourage everybody who contributes to the Registry to complete as much information as possible and to perform as much characterization as possible before sending us a sample.

We have many ribosome binding sites in our collection. You may want a strong RBS or one for your organism of choice. However, if you're not sure, you might want one that may other people have used, will just work in . At the time of writing, our most frequently used part is BBa_B0034. This ribosome binding site, first described my Michael Elowitz in 1999 has been used over 8000 times in the history of the Registry. You can find the number of uses for each part in the part quick reference box at the top of each part page.

The purpose of this page is to showcase the most highly-used parts in our collection so you may see how iGEMers on different teams approach making great parts.

Top 10 Most Used Coding Region Parts

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NameDescriptionLengthCreated byUsesStatusDoc
BBa_E0040 green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212720jcbraff875In stock35565
BBa_E1010 **highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)706Drew Endy593In stock46440
BBa_C0040tetracycline repressor from transposon Tn10 (+LVA)685June Rhee, Connie Tao, Ty Thomson, Louis Waldman.318In stock12575
BBa_C0051cI repressor from E. coli phage lambda (+LVA) 775Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross295In stock7093
BBa_C0012lacI repressor from E. coli (+LVA)1153Grace Kenney, Daniel Shen, Neelaksh Varshney, Samantha Sutton265In stock21550
BBa_C0062luxR repressor/activator, (no LVA?)781Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr247In stock42298
BBa_C0061autoinducer synthetase for AHL643Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr217In stock11645
BBa_E0030enhanced yellow fluorescent protein derived from A. victoria GFP723Caitlin Conboy and Jennifer Braff140In stock8152
BBa_I746916superfolder GFP coding sequence720Stefan Milde102In stock41280
BBa_K592009amilCP, blue chromoprotein669Lei Sun99In stock47318

Top 10 Most Used Promoters

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NameDescriptionLengthCreated byUsesStatusDoc
BBa_R0040TetR repressible promoter54 June Rhee, Connie Tao, Ty Thomson, Louis Waldman1081In stock25578
BBa_R0010promoter (lacI regulated)200 843In stock46722
BBa_J23100constitutive promoter family member35John Anderson786In stock83466
BBa_R0011Promoter (lacI regulated, lambda pL hybrid)55Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton598In stock23492
BBa_I0500Inducible pBad/araC promoter1210Sri Kosuri484In stock48514
BBa_J23119constitutive promoter family member35John Anderson442In stock31843
BBa_R0062Promoter (luxR & HSL regulated -- lux pR)55Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr374In stock77850
BBa_J23101constitutive promoter family member35John Anderson326In stock53848
BBa_R0051promoter (lambda cI regulated)49Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross284In stock9224
BBa_I719005T7 Promoter23Imperial 2007262In stock7912

Top 10 Most Used Ribosome Binding Sites

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NameDescriptionLengthCreated byUsesStatusDoc
BBa_B0034RBS (Elowitz 1999) -- defines RBS efficiency12Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. 5428In stock25043
BBa_B0030RBS.1 (strong) -- modified from R. Weiss15Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. 1001In stock17415
BBa_B0032RBS.3 (medium) -- derivative of BBa_003013Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. 907In stock10737
BBa_B0031RBS.2 (weak) -- derivative of BBa_003014Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.218In stock13140
BBa_J61100Ribosome Binding Site Family Member12John Anderson184In stock8254
BBa_Z0262Medium strength T7.2 RBS20Sriram Kosuri127Not in stock3181
BBa_J61101Ribosome Binding Site Family Member12John Anderson126In stock6164
BBa_Z0261Strong T7.2 RBS20Sriram Kosuri98Not in stock3179
BBa_K2066527B0034 with scar TACTAG18Joseph L Maniaci72Not in stock3316
BBa_K2406216MoClo RBS B003421Jack T. Suitor63Not in stock1422

Top 10 Most Used Terminators

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NameDescriptionLengthCreated byUsesStatusDoc
BBa_B0015double terminator (B0010-B0012)129Reshma Shetty4772In stock12466
BBa_B0010T1 from E. coli rrnB80Randy Rettberg1064In stock11502
BBa_B0012TE from coliphageT741Reshma Shetty1021In stock5501
BBa_B0014double terminator (B0012-B0011)95Reshma Shetty412In stock4662
BBa_B1006Terminator (artificial, large, %T~>90)39Haiyao Huang221In stock1859
BBa_K731721T7 terminator48Giacomo Giacomelli, Anna Depetris114In stock8059
BBa_B0025double terminator (B0015), reversed129Caitlin Conboy102In stock4033
BBa_B0011LuxICDABEG (+/-)46Reshma Shetty94In stock4374
BBa_K2933030T7 terminator48Jiahao Xie80In stock1226
BBa_K2486006ECK120029600 - Escherichia coli K-12 terminator90Cau Westmann and Gabriel Lencioni Lovate77Not in stock2080