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c-di-AMP

Part:BBa_K1045002:Design

Designed by: iGEM Team Göttingen 2013   Group: iGEM13_Goettingen   (2013-06-24)
Revision as of 16:54, 26 October 2013 by Kati (Talk | contribs) (→‎Design Notes)

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CFP Reporter under control of the c-di-AMP-dependent YdaO Riboswitch


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was amplified from Bacillus subtilis genomic DNA and ligated into the EcoRI and PstI digested pSB1C3 backbone (see BBa_K1045004). It was then further SpeI and EcoRI digested and ligated with the XbaI and EcoRI digested CFP (BBa_E0020). Altogether this composes BBa_K1045002.

Source

This composite part consists of parts either derived from PCR amplification of wild type B. subtilis chromosomal DNA (strain B. subtilis 168; Laboratory collection AG StĂŒlke, Department for General Microbiology, University Göttingen) or from the distribution kit 2013. For more information, see "Design Notes" above.

References

(1) Peter Y Watson & Martha J Fedor (2012) “The ydaO motif is an ATP-sensing riboswitch in Bacillus subtilis“, Nature Chemical Biology No. 8, pp. 963-965

(2) James W Nelson, Narasimhan Sudarsan, Kazuhiro Furukawa, Zasha Weinberg, Joy X Wang & Ronald R Breaker (2013) “Riboswitches in eubacteria sense the second messenger c-di-AMP“, Nature Chemical Biology, Advance Online Publication [doi: 10.1038/nchembio.1363]

(3) Yaara Oppenheimer-Shaanan, Ezequiel Wexselblatt, Jehoshua Katzhendler, Eylon Yavin & Sigal Ben-Yehuda (2011) “c-di-AMP reports DNA integrity during sporulation in Bacillus subtilis”, EMBO reports Vol. 12 No. 6, pp. 594-601