Part:BBa_K1159201

Splitted and engineered C-terminal FbaB for isopeptide bound formation (SpyTag) in RFC[25]

This part codes for a oligopeptide that is recognized and bound by a covalent isopeptide bound to a protein, called SpyCatcher. That catcher protein for this oligopeptdie can be found under BBa_K1159200. This part is flanked by RFC[25] pre- and suffix for further protein fusions.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Protein data table for BioBrick BBa_ automatically created by the BioBrick-AutoAnnotator version 1.0

Nucleotide sequence in RFC 25, so ATGGCCGGC and ACCGGT were added (in italics) to the 5' and 3' ends: (underlined part encodes the protein)
ATGGCCGGCGCTCATATT ... CCAACTAAGACCGGT
ORF from nucleotide position -8 to 45 (excluding stop-codon)

Amino acid sequence: (RFC 25 scars in shown in bold, other sequence features underlined; both given below)

1	MAGAHIVMVDAYKPTKTG*

Sequence features: (with their position in the amino acid sequence, see the list of supported features)

Spy-tag:

4 to 16

Amino acid composition:

Ala (A)	3 (16.7%)
Arg (R)	0 (0.0%)
Asn (N)	0 (0.0%)
Asp (D)	1 (5.6%)

Cys (C)	0 (0.0%)
Gln (Q)	0 (0.0%)
Glu (E)	0 (0.0%)
Gly (G)	2 (11.1%)

His (H)	1 (5.6%)
Ile (I)	1 (5.6%)
Leu (L)	0 (0.0%)
Lys (K)	2 (11.1%)

Met (M)	2 (11.1%)
Phe (F)	0 (0.0%)
Pro (P)	1 (5.6%)
Ser (S)	0 (0.0%)

Thr (T)	2 (11.1%)
Trp (W)	0 (0.0%)
Tyr (Y)	1 (5.6%)
Val (V)	2 (11.1%)

Amino acid counting

	Total number:	18
	Positively charged (Arg+Lys):	2 (11.1%)
	Negatively charged (Asp+Glu):	1 (5.6%)
	Aromatic (Phe+His+Try+Tyr):	2 (11.1%)

Biochemical parameters

	Atomic composition:	C₈₃H₁₃₆N₂₂O₂₄S₂
	Molecular mass [Da]:	1890.2
	Theoretical pI:	8.28
	Extinction coefficient at 280 nm [M^-1 cm^-1]:	1490 / 1490 (all Cys red/ox)

Plot for hydrophobicity, charge, predicted secondary structure, solvent accessability, transmembrane helices and disulfid bridges

Codon usage

	Organism:	E. coli	B. subtilis	S. cerevisiae	A. thaliana	P. patens	Mammals
	Codon quality (CAI):	good (0.76)	good (0.68)	excellent (0.81)	excellent (0.80)	excellent (0.84)	good (0.77)

Alignments (obtained from PredictProtein.org)

	SwissProt:	-
	TrEML:	-
	PDB:	-

Predictions (obtained from PredictProtein.org)

Subcellular Localization (reliability in brackets)

	Archaea:	cytosol (100%)
	Bacteria:	secreted (57%)
	Eukarya:	secreted (47%)

Gene Ontology (reliability in brackets)

	Molecular Function Ontology:	-
	Biological Process Ontology:	-

Predicted features:

	Disulfid bridges:	-
	Transmembrane helices:	-

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