Composite

Part:BBa_K1152016

Designed by: Ralf Beer, Konrad Herbst, Nikolaos Ignatiadis   Group: iGEM13_Heidelberg   (2013-09-20)
Revision as of 03:44, 5 October 2013 by RBeer (Talk | contribs)

IndC Indigoidine Synthetase device synT1 (engineered)

This engineered version of indC BBa_K11520013 contains a synthetic T-domain instead of its native T-domain. It still is capable to produce the blue pigment indigoidine, as OD-measurements showed.

In the multiple sequence alignment showed below, this variant of indC is named synT1.

Multiple Sequence Alignment of the synthetic T-domains we introduced to indC

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 4087
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1467
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2730


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