Composite

Part:BBa_K1152013

Designed by: Ralf Beer, Konrad Herbst, Nikolaos Ignatiadis   Group: iGEM13_Heidelberg   (2013-09-20)
Revision as of 02:16, 5 October 2013 by RBeer (Talk | contribs)

IndC Indigoidine Synthetase device Nonribosomal Peptide Synthetase that synthesizes the blue pigment indigoidine. The gene was amplified from P. luminescens laumondii TT01 (DSM15139).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 4087
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1467
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2730

Restriction digest of BBa_K1152013 with EcoRI, XbaI, SpeI and PstI


[edit]
Categories
Parameters
None