Device
Part:BBa_K1150035
Designed by: Ilona Blank Group: iGEM13_Freiburg (2013-09-17)
uniCAS RNAimer to EMX1
RNAimer (EMX1 target) | |
---|---|
Function | Targeting EMX1 with Cas9 |
Use in | Mammalians |
RFC standard | RFC 25 |
Backbone | pSB1C3 |
Submitted by | [http://2013.igem.org/Team:Freiburg Freiburg 2013] |
This device derived from BBa_K1150034 can be used in combination with all devices with dCas9. It contains a DNA sequence coding for a crRNA that binds complementary to EMX1 target (GGAAGGGCCTGAGTCCGAGCAGAAGAAGAA).
For more information about dCas9 devices [http://2013.igem.org/Team:Freiburg klick here].
Biology and Usage
Freiburg 2013 used this plasmid to test the efficiency of target EMX1 by activating or repressing a SEAP reporter gene.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 224
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 216
[edit]
Categories
Parameters
None |