Help:Plasmid backbones
Plasmids are circular, double-stranded units of DNA that replicate within a cell independent of the chromosomal DNA within the cell. They are often acquired by cells and can bestow the benefit of antibiotic resistance or protein production. Plasmids are used to incorporate biobrick devices and systems within cells via transformation.
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Antibiotic ResistanceBy incorporating an antibiotic-resistance gene on a plasmid, engineered-cells are able to be selected for using the appropriate antibiotic. The most common resistances are ampicillin ("Amp"), kanomycin ("Kan"), tetracycline ("Tet") and chloramphenicol ("Chlor"). For more information on which plasmids carry these resistances, click the nomenclature documentation section. All of our plasmids are available through the use of BBa_P1010. (Information on how to use that part is available on its part page) |