Part:BBa_K1150032
uniCAS UV Light Switch Part II - Histone Modifier
| CMV:COP1-Linker-G9a-NLS:BGH | |
|---|---|
| Function | Histone modification domain
of UVB light induced gene expression control |
| Use in | Mammalians |
| RFC standard | RFC 25 |
| Backbone | pSB1C3 |
| Submitted by | [http://2013.igem.org/Team:Freiburg Freiburg 2013] |
Introduction
This part contains the protein COP1 fused to the Methyl-Tranferase G9a. It's an interaction partner of dCas9-UVR8 and part of the UVB light controlled mechanism to repress gene expression. You will be able to repress any desired gene by a short UV-light pulse.
For more information and experimental data have a look at our [http://2013.igem.org/Team:Freiburg/Project/induction#light light] and [http://2013.igem.org/Team:Freiburg/Project/effector#epigenetics epigenetic] project page.
Biology & Usage
COP1 is a protein that was first described in the model organism Arabidopsis thaliana. It will bind to the UV-light receptor UVR8 after illumination [1]. Thereby recruiting G9a to its target locus. Subsequently the local DNA-expression will be repressed by DNA-methylation. For this UVB light controled gene repression system you need a combination of these BioBricks: Cas9-UVR8, COP1-G9a and RNaimer.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 576
Illegal BglII site found at 2231 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1373
Illegal BsaI.rc site found at 1450
Illegal SapI.rc site found at 962
References
[1] Müller, K.; Engesser, R.; Schulz, S.; Steinberg, T.; Tomakidi, P.; Weber, C. C. et al. (Nucleid Acids Research 2013): Multi-chromatic control of mammalian gene expression and signaling
| None |