Device

Part:BBa_K1150036

Designed by: Ilona Blank   Group: iGEM13_Freiburg   (2013-09-17)
Revision as of 13:09, 4 October 2013 by Ilona (Talk | contribs)

uniCAS RNAimer to target VEGF1

RNAimer (VEGF1 target)
Function Targeting VEGF1 with Cas9
Use in Mammalians
RFC standard RFC 25
Backbone pSB1C3
Submitted by [http://2013.igem.org/Team:Freiburg Freiburg 2013]

This device can be used in combination with dCas9. It contains a DNA sequence that transcripts a crRNA that binds complementary to VEGF1 target (TTAAAAGTCGGCTGGTAGCGGGGAGGATCG).

Biology and Usage

Freiburg 2013 used this plasmid to test the efficiency of target VEGF3. Therefore this target was also cloned in front of a promoter, that on its part is located in front of a SEAP reporter gene.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 224
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 216

Literature

[edit]
Categories
Parameters
None