Part:BBa_K1104206

AhpCpD1

AhpCpD1

We improve the function of a BioBrick Part: ahpC promoter(K362001)designed by [http://2010.igem.org/Team:KIT-Kyoto/Parts 2010 KIT-Tokyo team] into four versions.On PartRegistry, the complex part(according to [http://ecocyc.org/ECOLI/new-image?object=EG11384 Ecocyc]) composition contains hybrid promoters, shared TFBS (Transcription Factor Binding Site), and reverse promoter DsbG.

Improvement of ahpC promoter(Part:K362001)

OxyR binding to the ahpC-proximal site leads to the induction of both dsbG and ahpC transcripts, while OxyR binding to the dsbG-proximal site leads to the induction of a second ahpC transcript. This transcript of ahpC and the transcript of dsbG overlap by over 100 nucleotides.

The intergenic region between dsbG and ahpC carries two binding sites for OxyR, a dsbG-proximal site, located 54 bp upstream of the dsbG start codon; an ahpC-proximal site, located 290 bp upstream of the dsbG start codon.

Usage and Biology

There are two TFBSs between AhpCp1 and reverse DsbGp. OxyR binding to the ahpC-proximal site leads to the induction of both dsbG and ahpC transcripts.

Once ROS(Reactive Oxygen Species) oxidizes OxyR, making OxyR interact with the TFBS between AhpCp1 and DsbGp, dual-directional transcription will occur. It can be take advantages of by certain special circuit design.

Related Parts

ahpC(Part:BBa_K362001)
- AhpCp1000(Part:BBa_K1104203)
  - Part:BBa_K1104243: AhpCp1000+E0840
- AhpCp2D1(Part:BBa_K1104204)
  - Part:BBa_K1104244: AhpCp2D1+E0840
- AhpCp2(Part:BBa_K1104205)
  - Part:BBa_K1104245: AhpCp2+E0840
- AhpCpD1(Part:BBa_K1104206)
  - Part:BBa_K1104246: AhpCpD1+E0840
- AhpCp1(Part:BBa_K1104207)
  - Part:BBa_K1104247: AhpCp1+E0840
- DsbGp(Part:BBa_K1104208)
  - Part:BBa_K1104248: DsbGp+E0840

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]