Coding

Part:BBa_K1150006

Designed by: Freiburg 2013   Group: iGEM13_Freiburg   (2013-09-16)
Revision as of 11:29, 28 September 2013 by Ilona (Talk | contribs)

UVR8

dCas9
Function UVB receptor
Use in Mammalians and plants
RFC standard RFC 25
Backbone pSB1C3
Organism Arabidopsis thaliana
Source Konrad Müller
Submitted by [http://2013.igem.org/Team:Freiburg Freiburg 2013]

Sunlight is essential for plants: Not only as an energy source but also as information for their growth cycle. Therfore they need receptors to detect different light wavelengts. Ultraviolet B (UVB) light can be perceived with photoreceptor UV resistance Locus 8 (UVR8) [1]. This UVR8 receptor derives drom Arapidopsis thaliana [2].

Usage and Biology

In the absecne of UVB light UVR8 forms homodimers. This is its closed configuration. When UVB light is perceived UVRB monomerizes and changes into its open configuration. Then its able to recruit COP1.

In the absecne of

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 37
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 52

Literature

[1] Rizzini L., Favory J.-J., Cloix C., Faggionato D., O'Hara A., Kaiserli E., Baumeister R., Schäfer E., Nagy F., Jenkins G. I., Ulm R. (2011). Perception of UV-B by the Arabidopsis UVR8 protein

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Categories
Parameters
None