Coding

Part:BBa_K1129037

Designed by: UBC iGEM 2013   Group: iGEM13_British_Columbia   (2013-09-16)
Revision as of 03:08, 28 September 2013 by Fisal (Talk | contribs)

4-coumarate-CoA ligase (4CL) under arabinose promoter

Arab.+rbs+4-CL+term.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
    Illegal NheI site found at 1242
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1809
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1441



The 4-coumarate-CoA ligase (4CL) enzyme catalyzes the ligation of a CoA group to the end of the molecule. The addition of this CoA provides better kinetics for a variety of downstream reactions. 4CL is also a member of the phenylpropanoid pathway and is found in a wide variety of organisms that partake in the degradation of lignin. Here we harness the 4CL to provide better kinetics for reduction of cinnamic acid to cinnamaldehyde.

Resources

http://www.uniprot.org/uniprot/Q1H8P3

http://en.wikipedia.org/wiki/4-Coumarate-CoA_ligase

http://www.genome.jp/kegg-bin/show_pathway?map00940

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Categories
Parameters
None