Regulatory
HIP

Part:BBa_K1166000

Designed by: Luis Mario Leal Garza   Group: iGEM13_TecMonterrey   (2013-09-17)
Revision as of 02:44, 28 September 2013 by LuisFernandoCG (Talk | contribs)

FNR-HIP1

HIP-1 drives gene expression under both acute and chronic hypoxia, but not under normoxia (Mengesha, et al., 2006). This part is composed of the HIP-1 promoter with the FNR expression cassette upstream for a stronger hypoxic regulation, this promoter will start translation in coding sequences 3’ downstream in a hypoxic environment.

Characterization

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HIP-1 characterization.jpg

References

Mengesha A, Dubois L, Lambin P, Landuyt W, Chiu RK, Wouters BG, Theys J. (2006). Development of a flexible and potent hypoxia-inducible promoter for tumor-targeted gene expression in attenuated Salmonella. Cancer Biol Ther. 5(9):1120-8.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None