Reporter

Part:BBa_K1100015:Design

Designed by: Chenxi He, Jiadong Ni   Group: iGEM13_Fudan   (2013-09-13)
Revision as of 23:53, 27 September 2013 by Nzy (Talk | contribs) (→‎Design Notes)

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R0010 with insulator and sfGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To test the stochastic dynamics of the constitutive promoters by means of measuring fluorescence intensity of sfGFP using flow cytometry. The plasmid map of K1100015.png

Source

R0010 from Kit, Insulator: Csy4 loci via PCR, sfGFP from Ding Yu lab at Fudan University

References

Qi L, Haurwitz R E, Shao W, et al. RNA processing enables predictable programming of gene expression[J]. Nature biotechnology, 2012, 30(10): 1002-1006. Haurwitz R E, Jinek M, Wiedenheft B, et al. Sequence-and structure-specific RNA processing by a CRISPR endonuclease[J]. Science, 2010, 329(5997): 1355-1358.