Coding

Part:BBa_K1054001

Designed by: Jiasheng Wang   Group: iGEM13_ZJU-China   (2013-09-10)
Revision as of 20:20, 27 September 2013 by Stargazin (Talk | contribs)

protein E of Phage phiX174 under pBAD

Prontein E is a membrane protein that inhibits MraY, an enzyme required for murein synthesis, and promotes cell lysis in a manner analogous to cell wall synthesis inhibitors like penicillin. However, it will keep the periplasmic space of Gram-negative strain intact. In this part, protein E will be expressed when L-arabinose is available.

500px-ZJU-ghostsensor-result-1.png

The growth curve of transformed E.coli cells treated with different concentrations of L-arabinose varying from 0.2% to 1%. MgSO4 has been reported to allow the expression of protein E but inhibit lysis. MgSO4 was added 30min before the induction according to previous works by others.


330px-ZJU-ghostsensor-result-2.png 330px-ZJU-ghostsensor-result-3.png

Left: SEM picture of protein E lysed E.coli.

Right: SEM picture of protein E lysed E.coli with MgSO4 protocol. The tunnel is relatively bigger than the left one.



Refereces [1] S. Resch et al., Aqueous release and purification of poly(b-hydroxybutyrate) from Escherichia coli, Journal of Biotechnology 65 (1998) 173–182

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//function/celldeath
Parameters
n/aprotein E of Phage phiX174 under pBAD