Signalling

Part:BBa_K1166003

Designed by: Luis Mario Leal Garza   Group: iGEM13_TecMonterrey   (2013-09-17)
Revision as of 18:33, 27 September 2013 by LuisFernandoCG (Talk | contribs)

His-TAT

A biobrick that includes the TAT signal peptide that can be fused to any protein for an easy internalization into mammalian cells. It includes a 6x His-tag for easy purification. TAT is a cell penetrating peptide (CPP) derived from the transactivator of transcription from HIV and has been used to internalize a range of large and small molecules from peptides, DNA and antibodies, to liposomes.


References

Vivès E, Brodin P, Lebleu B. (1997). A truncated HIV-1 Tat protein basic domain rapidly translocates through the plasma membrane and accumulates in the cell nucleus. J Biol Chem. 272(25):16010-7

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None