Part:BBa_K1074006:Experience

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••• USTC_China iGEM13

In our project, we construct other four gene circuits based on this part. In these gene circuits, GFP is replaced by HBsAg, Ag85b, PAD4, LTB respectively. We transformed the recombinant Plasmids both in BL21 and Bacillus Subtilis WB800N and induced expression with IPTG . Results analyzed by SDS-page , mass spectrum and ELISA were proved positive. But proteins expressed in WB800N were far less than those in BL21. The antigenicity and transdermal function of N-terminal TD1 modified antigen HBsAg was also tested positive by ELISA. figure 1 SDS-PAGE of N-terminal TD1 modified GFP figure 2 SDS-PAGE of N-terminal TD1 modified LTB and HBsAg figure 3 Fluorescence detection of N-terminal TD1 modified GFP expression with and without IPTG induction figure 4 ELISA test transdermal function of N-terminal TD1 modified antigen HBsAg

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