Regulatory
pLas + RBS

Part:BBa_K1073003:Experience

Designed by: iGEM Team Braunschweig 2013   Group: iGEM13_Braunschweig   (2013-09-16)
Revision as of 07:17, 27 September 2013 by Kerstin K (Talk | contribs) (→‎User Reviews)

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This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1073003

The iGEM Team Braunschweig 2013 used this device in combination with an ampicillin resistance gene (BBa_K1073000) to induce growth by LasR and N-3-oxododecanoyl-HSL of different E.coli strains in ampicillin containing media.

User Reviews

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iGEM Team Braunschweig 2013

Using this device in combination with an ampicillin resistance gene cells showed a higher growth rate in ampicillin containing media when the promoter was induced. The device shows leakiness to a constant low degree in yeast extract complex media. Background activity of the genes downstream of the promoter was detected in all experiments. However activity was much higher when the promoter was induced by LasR and N-3-oxododecanoyl-HSL.

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