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Part:BBa_K1228008

Designed by: Zhang SW   Group: iGEM13_HZAU-China   (2013-09-09)
Revision as of 07:02, 27 September 2013 by Zhevol (Talk | contribs) (→‎Experiment)

38aa with T1 terminator and Constitutive promoter veg

Human lysozyme (hLZ), the actual protein in breast milk,exhibits microbicidal activity to various degrees against several bacterial strains.The HLH peptide and its N-terminal helix (H1) were significantly the most potent bactericidal to Gram-positive and Gram-negative bacteria.Evidence shows that HLH peptide and its N-terminal helix (H1) kill bacteria by crossing the outer membrane of Gram-negative bacteria via self-promoted uptake and are able to dissipate the membrane potential-dependent respiration of Gram-positive bacteria.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Experiment

In order to distinguish whether the B. Subtilisexpress our objective protein or not,we used the method of in-vitro bacterial inhibition zone to observe the anti-bacterial effect.We added 100 ul B. Subtilis liquid medium into the hole. The inhibition zone results showed that gram-positive bacterium was sensitive to 38aa.

[edit]
Categories
//chassis/prokaryote/bsubtilis
Parameters
n/a38aa with T1 terminator and Constitutive promoter veg